Dengue IgG Capture ELISA Kit

Catalog Number: E-DEN02G
Availability Notice :
This product is not for sale or distribution in the United States and may not be available in all countries.
Please contact Inverness Medical or your local Panbio distributor to confirm availability.

Product Overview :
The Dengue IgG Capture ELISA is a qualitative enzyme-linked immunosorbent assay designed for the detection of IgG antibodies against dengue virus serotypes 1, 2, 3, and 4 in human serum.
This assay is intended to support the clinical laboratory diagnosis of secondary dengue virus infection. When used in combination with the Dengue IgM Capture ELISA, it enables the presumptive differentiation between primary and secondary dengue infections with a high level of confidence.
The Dengue IgG Capture ELISA provides a reliable and standardized alternative to the traditional Hemagglutination Inhibition Assay (HAI) for the serological diagnosis of secondary dengue infection.
The IgG cut-off is optimized to detect the elevated IgG antibody levels characteristic of secondary dengue cases.
Intended Use:
Aid in the diagnosis of secondary dengue virus infection
Suitable for use in dengue-endemic regions
Designed for professional laboratory use
Recommended for use alongside the Dengue IgM Capture ELISA to distinguish primary from secondary infection
Key Benefits:
High specificity for dengue virus IgG antibodies
Optimized IgG cut-off for secondary dengue detection
Standardized and reproducible ELISA methodology
Ready-to-use, color-coded reagents
Break-apart wells for flexible testing
Compatible with standard ELISA microplate readers
Technical Information
Assay format: Capture ELISA
Sample type: Serum
Total incubation time: 2 hours 10 minutes
Wells: Break-apart microplate
Reagents: Ready to use
Kit size: 96 tests
Companion assay: Dengue IgM Capture ELISA (E-DEN01M)
Clinical Performance :
Primary Dengue :
IgM Sensitivity : 54/57 (94.7%)
IgG Sensitivity : N/A
Secondary Dengue* :
IgM Sensitivity : 64/115 (55.7%)
IgG Sensitivity : 52/54 (96.3%)
Serological Specificity:
Negative Samples :
IgM Specificity : 83/83 (100%)
IgG Specificity : 125/125 (100%)
Primary Dengue :
IgM Specificity : N/A
IgG Specificity : 32/35 (91.4%)
* Samples positive for secondary dengue infection by HAI (titre ≥ 2560) Data extracted from Dengue IgM and Dengue IgG Capture ELISA Instructions for Use.
Cross-Reactivity The Dengue IgG Capture ELISA demonstrates minimal cross-reactivity:
West Nile Virus 0 /10
Malaria 0 / 10
Scrub Typhus 0 / 7
Leptospira 2 / 12
Hepatitis A 0 / 6
Rheumatoid Factor 0 / 10
Anti-Nuclear Antibodies 1 / 10
Total 3 / 65

Publications
Cuzzubbo, A., et al. (1997). Commercial Assays for the Serological Diagnosis of Dengue Infection. Arbovirus Research in Australia. 7:56-60. Queensland Institute of Medical Research.

Cuzzubbo, A., et al (1998) Detection of Specific Antibodies in Saliva during Dengue Infection. Journal of Clinical Microbiology Dec. 1998:3737-3739

Cuzzubbo, A., et al. (1999) Comparison of PanBio Dengue Duo Enzyme-Linked Immunosorbent Assay (ELISA) and MRL Dengue Fever Virus Immunoglobulin M Capture ELISA for Diagnosis of Dengue Virus Infections in Southeast Asia. Clinical and Diagnostic Laboratory Immunology Sep. 1999:705-712

Lam, K., et al (2000) Evaluation of a Capture Screening Enzyme-Linked Immunosorbent Assay for Combined Determination of Immunoglobulin M and G Antibodies Produced during Dengue Infection. Clinical and Diagnostic Laboratory Immunology Sep. 2000:850-852

Porter, K., et al (1999) Evaluation of a Commercially Available Immunoglobulin M Capture Enzyme-Linked Immunosorbent Assay Kit for Diagnosing Acute Dengue Infections. Clinical and Diagnostic Laboratory Immunology Sep. 1999:741-744

Sang, C., et al (1998) Evaluation of a Commercial Capture Enzyme-Linked Immunosorbent Assay for Detection of Immunoglobulin M and G Antibodies Produced during Dengue Infection. Clinical and Diagnostic Laboratory Immunology Jan. 1998: 7-10

Vaughn, D., et al (1999) RAPID SEROLOGIC DIAGNOSIS OF DENGUE VIRUS INFECTION USING A COMMERCIAL CAPTURE ELISA THAT DISTINGUISHES PRIMARY AND SECONDARY INFECTIONS. Am. J. Trop. Med. Hyg. 60(4): 693-698

Vazquez, S., et al (2007) Evaluation of immunoglobulin M and G capture enzyme-linked immunosorbent assay Panbio kits for diagnostic dengue infections. Journal of Clinical Virology 39:194-198